Contact an Account ManagerResolution of a-substituted amino acid enantiomers by HPLC after derivatization with a chiral adduct of o-phthalaldehyde. Application to glutamic acid analogues
Summary
Type: HPLC
Application #: 105380
Catalog#: 720014.40 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4x250mm
Detection: fluorescence, excitation 345nm, emission 445nm
Conditions: eluent: 0.1M KH2PO4 buffer, adjusted to pH 7.5 with NaOH, containing 20% methanol
flow rate 1ml/min
Authors: Maurs M
Source: J Chromatogr 440 (1988) 209 - 215
Details
Legend: Fig. 4. Separation of diastereoisomeric adducts formed from 1S- (1) and 1R- (2)
cis-1-amino-1,3-dicarboxycyclopentane and 1S- (3) and 1R- (4)
trans-1-amino-1,3-dicarboxycyclopentane.